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KMID : 0624620100430030182
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BMB Reports
2010 Volume.43 No. 3 p.182 ~ p.187
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CMTM5-v1, a four-transmembrane protein, presents a secreted form released via a vesicle-mediated secretory pathway
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Li Henan
Guo Xiaohuan
Shao Luning
Plate Markus
Mo Xiaoning
Wang Yu
Han Wenling
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Abstract
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The CKLF-like MARVEL transmembrane domain-containing family (CMTM) is a novel family of proteins linking classical chemokines and the transmembrane 4 superfamily (TM4SF). Our earlier studies indicated several CMTM members (such as CKLF1 and CMTM2) have a secreted form. This is the first report of the secreted form of CMTM5-v1, the major RNA splicing form of CMTM5, which is produced as small vesicles (＜100 nm diameter) and floats at a peak density of 1.19 g/ml on continuous sucrose gradients. CMTM5-v1 has no obvious co-localization with CD63 or Golgi complex. In addition, brefeldin A but not wortmannin can inhibit the secretion of CMTM5-v1. Our results suggest that CMTM5-v1 might be secreted via a different vesicle-mediated secretory pathway, which will be helpful for the studies of vesicle-mediated secretion and MARVEL domain-containing proteins.
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KEYWORD
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CMTM, Secretion, TM4SF, Vesicles
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